Tubulin Antibody – MCA-4E4
- Clonality : Mouse Monoclonal
- Applications : WB | IF/ICC | IHC
- Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
310.00$ – 1,660.00$ CAD
0 in stock (expect 7-14 days for delivery)
Encor Mouse Monoclonal to beta tubulin
Tubulins are a major class of cytoskeletal proteins and divided into five distinct classes, namely α, β, γ, δ and ε tubulins. The most abundant members of the tubulin family are the α and β-tubulins and are the major components of cytoplasmic microtubules. The various subunits have molecular weights of approximately 55 kDa and are 50% identical to one another at the protein sequence level. Microtubules are assembled from stable dimer of one α and one β subunit, and polymerization from dimers to assembled microtubules requires GTP. Microtubules are involved in a number of essential cellular functions including the maintenance of cell shape, transport, motility, cell signaling and mitosis (1). β tubulin is regarded as a “house keeping” protein which is generally not altered much in expression as a result of experimental manipulations. As a result antibodies to β tubulin are widely used as loading controls in western blotting as a standard by which the levels of other proteins may be measured. The important role of microtubules in cell division makes them a desirable target for the development of chemotherapeutic agents directed against rapidly dividing cancer cells (2). MCA-4E4 was raised against tubulin purified from pig brain and reacted with recombinant β-tubulin (Abcam), but not recombinant α-tubulin (Abnova) by ELISA and dot blots.
HGNC name(s) : TUBB
Host : Mouse
Clonality : Monoclonal
ID : EnCor Biotechnology Tubulin 4E4
Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
Isotype : IgG2a
Conjugation : none
Immunogen : Purified bovine
Mass of detected protein : 55 kDa
Uniprot ID : P07437
KGNC name : TUBB
RRID # : AB_2492290
Purification : Affinity purified at 1 mg/mL
Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Validated applications : WB | IF/ICC | IHC
WB:1:5 000-1:10 000, ICC/IF and IHC:1:1000-1:5 000.
1. Nogales E. Structural insight into microtubule function. Annu Rev Biophys Biomol Struct 30:397–420 (2001).
2. Perez EA. Microtubule inhibitors: Differentiating tubulin-inhibiting agents based on mechanisms of action, clinical activity, and resistance. Mol Cancer Ther 8:2086-2095 (2009).
50 ul, 100 ul, 500 ul