TransStart TIP Green qPCR SuperMix – AQ141 (with EvaGreen)

TransStart Tip Green qPCR SuperMix

TransStart TIP Green qPCR SuperMix is a ready-to-use qPCR cocktail containing a novel TransStart® TipTaq DNA Polymerase (three different blocking proteins used for blocking template and primers, one special chemical binding to DNA polymerase to block its activity), optimized double cation buffer, EvaGreen® fluorescence dye, dNTPs, PCR Enhancer and PCR stabilizer. qPCR SuperMix is provided at 2x concentration and can be used at 1x concentration by adding template, primer, passive reference dye (optional) and ddH₂O.

TransStart TIP Green qPCR SuperMix is also available ain 5x and 10x formulations (special request)

What’s New in the newly Optimized version of AQ141?

• Amplification efficiency for most tested genes is equal to competitor Bio-Rad SsoFast™ EvaGreen® Supermix (1725201) and Takara SYBR® Fast qPCR Mix (RR430A). Results for different genes may vary.
• Improved standard curve linearity than 2017 batches.
• Novel EvaGreen® dye now replaces SYBR green I – the same as Bio-Rad (2018 batches are slightly yellow while the older versions were translucid).
• Enhanced fluorescent signal : Much stronger than competitor Takara and 2017 batches but slightly weaker than BioRad.

Advantages of TransStart Tip Green qPCR SuperMix:

• A combination of chemical blocking technique and TransStart® “hot start” technique to achieve complete blocking. Compared with double blocking TransStart® TipTaq, this method provides higher sensitivity, higher specificity, better amplification.
• Double cation (K+, NH4+) buffer enhances the specificity and reduces primer-dimer formation.
• Passive reference dyes are suitable for different qPCR instruments (normalize the fluorescent signal between reactions).

Kit Content:

• 2x TransStart TIP Green qPCR SuperMix
• Passive Reference Dye I and II (50x)
• ddH2O

Reference Dye compatibility

• Passive Reference Dye I (50×)
  ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
  ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
  Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

Manual and Datasheet:

TransStart® TIP Green qPCR SuperMix