TransScript® II One-Step RT-PCR SuperMix (+dye) – AH411
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TransScript® II One-Step RT-PCR SuperMix (+dye)
TransScript® II One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript® II RT and TransTaq® HiFi DNA Polymerase are used in the kit.
Advantages of TransScript® II One-Step RT-PCR SuperMix
- High efficiency cDNA synthesis with TransScript® II One-Step Enzyme Mix and 2xOne-Step Reaction Mix, followed by PCR amplification with resulting cDNA. Simple procedure helps to minimize contamination.
- Amplification product contains base “A” at 3’ end. Purified product is well suitable for cloning into pEASY®-T vectors.
- Amplified fragment up to 8 kb.
- RNase inhibitor included in the mix.
Manual and Datasheet