TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal) – AH341

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5x concentration and used at 1x concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed in one tube. cDNA synthesis can be carried at temperature between 42 and 55°C. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds. The resulting cDNA are suitable for qPCR and not for regular PCR.

Advantages of TransScript® II All-in-One

• Simultaneous genomic DNA removal and cDNA synthesis in one tube.
• The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.
• cDNA synthesis can be carried at temperature between 42 and 55°C.
• Multiple copy and low-copy gene detection.
• GC-rich or complex secondary structure RNA template.

Manual and Datasheet:

AH341 – TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR

https://www.civicbio.com/wp-content/uploads/2015/09/TransScript®-II-All-in-One-First-Strand-cDNA-Synthesis-SuperMix-for-qPCR.pdf

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