TransScript® Green miRNA Two-Step qRT-PCR SuperMix – AQ202
TransScript® Green miRNA Two-Step qRT-PCR SuperMix
TransScrip® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for miRNA detection.High efficient poly(A) tail addition and first-strand cDNA synthesis are performed byTransScript® miRNA RT Enzyme Mix (containing tailing enzyme and RT enzyme) and 2x TS miRNA Reaction Mix. TransStart® Top Green qPCR SuperMix is provided for miRNA detection. This kit is suitable for detection of miRNA-containing samples like total RNA or small RNAs.
- Optimal ratio of tailing enzyme to RT enzyme and optimized reaction buffer, so as to ensure the efficiency of miRNA reverse transcription.
- One-step completion of Poly(A) tailing addition and cDNA synthesis in one tube.
- TransStart® Top Green qPCR SuperMix is of powerful amplification capability, high specificity, high sensitivity and makes data accurate.
- Passive reference dyes are suitable for different qPCR instruments (normalize the fluorescent signal between reactions).
Manual and Datasheet:
RT (20 x 20 ul rx) + PCR (500 x 20 ul rx)
Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.
Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...
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