TransNGS Stranded RNA-Seq Library Prep Kit for Illumina – KP301
464.00$ – 4,340.00$ CAD
Stranded RNA Seq Library Prep Kit for Illumina
TransNGS Stranded RNA-Seq Library Prep Kit for Illumina is designed to prepare Stranded RNA libraries compatible with the Illumina® sequencers. Differently from conventional stranded RNA seq library prep kits, the TransNGS Stranded RNA seq kit generates cDNA using random hexamer with a 3’-Adaptor. Subsequently, the 5’-Adaptor is ligated at the 3’-end of the first-strand cDNA, bypassing the need for 2nd strand synthesis and digestion. Then, end-repair and dA-tailing are performed. As a result, only information from the 1st strand cDNA is preserved. This kit is tested and promised to provide stable and reproducible cDNA libraries, by using either total RNA samples, rRNA-depleted samples, mRNA purified samples or lncRNA samples.
- User-friendly and fast with minimal hands-on time
- High efficiency with minimal amount of input DNA
- Operation is simple and fast
- Gene expression analysis
- Single–nucleotide variation (SNV) discovery
- Splice junction and gene fusion identification
- Whole transcriptome analysis
- Characterization of non-polyadenylated RNAs, including non-coding and immature RNAs
|Components||KP301-01 (6rxns)||KP301-02 (24rxns)||KP301-03 (96rxns)|
|2×RNA-Seq RT Reaction Mix||60 μl||240 μl||4×240 μl|
|RNA-Seq RT Enzymes Mix||6 μl||24 μl||4×24 μl|
|RNA Digestion Mix||6 μl||24 μl||4×24 μl|
|cDNA Binding Buffer||180 μl||720 μl||4×720 μl|
|5′-Adaptor||6 μl||24 μl||96 μl|
|2×RNA-Seq Ligation Mix||150 μl||600 μl||4×600 μl|
|RNA-Seq Ligase||30 μl||120 μl||480 μl|
|RNA Ligation Stop Buffer||200 μl||800 μl||4×800 μl|
|TransNGSTM Library Amplification SuperMix||150 μl||600 μl||4×600 μl|
|Library Elution Buffer (LEB)||1.2 ml||5 ml||4×5 ml|
|RNase-free Water||1 ml||1 ml||5 ml|
Cat. No. KP301
Storage: at -20°C for one year
RNA Input Recommendations
Starting Materials: 10 ng – 1 μg total RNA treated with rRNA depletion, 0.1 μg – 4 μg total RNA treated with mRNA purification and lncRNA.
Requirements: high quality RNA, which is recommended to use an Agilent 2100 Bioanalyzer to analyze the total RNA. The RIN (RNA integrity number) value should be ≥ 7.0. Using degraded total RNA for library construction will lead to in 3’ bias in RNA-seq. The ratio of OD260/OD280 should be between 1.8 and 2.1.
Reagents not included in the kit
- 80% ethanol (freshly prepared);
- DNA size selection beads like MagicPure Size Selection DNA Beads (TransGen, EC401);
- Index Primers : TransNGS Single Index Primers Kit for Illumina® (TransGen, KI201-KI221) or Dual Index Primers Kit for Illumina® (TransGen, KI231).
Workflow for Stranded RNA library prep
Manual & Datasheet
6 rx, 24 rx, 96 rx
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Product Reviews for TransNGS Stranded RNA-Seq Library Prep Kit for Illumina – KP301
464.00$ – 4,340.00$ CAD