TransNGS Index Primer Kits for Illumina®
Single Index Primer Kits KI201, KI211 and KI221 (Set 1, Set 2 and Set 3 respectively)
TransNGS® Single Index Primer Kits for Illumina® are composed of 3 different sets of primers. Each set of primers contains 12 unique barcoded i7 primers and one NGS Universal Primer for Illumina®.
Dual Index Primer Kit KI231
For multiplex next-generation sequencing library preparations, please use the TransNGS® Dual Index Primer Kit for Illumina®. Dual indexes are ideal for multiplex next-generation sequencing library preparations for Illumina sequencers. The Dual Index Primer kit provides 8 different i5 primers (i501-i508) and 12 different i7 primers (i701-i712). In total, 96 uniquely dual-indexed libraries can be prepared using this kit.
Dual Index Primer Kit KI231
TransNGS Index Primers (384) Kit for Illumina® is suitable for multiplex library preparation for next-generation sequencing on the Illumina platform. This kit contains 16 different i5 primers (i509-i524) and 24 different i7 primers(i713-i736). 384 uniquely dual-indexed libraries can be prepared using this kit.
TransNGS™ Index Kits for Illumina® are compatible with other TransNGS series products and with other NGS Library Prep Kits from different vendors.
TransNGS Index Primer Sets for Illumina®
| Component | KI201-01 | KI201-02 | KI211-01 | KI211-02 | KI221-01 | KI221-02 | KI221-01 | KI221-02 |
| Universal Primer (10 μM) | 120 μl | 240 μl | 120 μl | 240 μl | 120 μl | 240 μl | ||
| Index 1 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 2 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 3 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 4 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 5 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 6 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 7 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 8 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 9 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 10 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 11 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 12 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 13 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 14 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 15 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 16 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 18 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 19 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 20 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 21 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 22 Primer(10 μM) | 10 μl | 20 μl | ||||||
| Index 23 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 25 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 27 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 17 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 24 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 26 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 28 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 29 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 30 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 31 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 32 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 33 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 34 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 35 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 36 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 37 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 38 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 39 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 40 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 41 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 42 Primer(10 μM) | 10 μl | 20 μl | ||||||
| Index 43 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 44 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 45 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 46 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 47 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Index 48 Primer (10 μM) | 10 μl | 20 μl | ||||||
| Dual Index Primer Set | KI221-01 | KI221-02 | ||||||
| NGS i501 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i502 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i503 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i504 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i505 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i506 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i507 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i508 Primer (10 μM) | 15μl | 30 μl | ||||||
| NGS i701 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i702 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i703 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i704 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i705 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i706 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i707 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i708 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i709 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i710 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i711 Primer (10 μM) | 10 μl | 20 μl | ||||||
| NGS i712 Primer (10 μM) | 10 μl | 20 μl |
Dual Index Primer Set for 384 unique barcodes (KI241)
| Component | KI241-01 (96 rxns) | KI241-02 (384 rxns) | |
| i5 Primer | TransNGS® i509 primer | 15 μl | 60 μl |
| TransNGS® i510primer | 15 μl | 60 μl | |
| TransNGS® i511 primer | 15 μl | 60 μl | |
| TransNGS® i512 primer | 15 μl | 60 μl | |
| TransNGS® i513 primer | 15 μl | 60 μl | |
| TransNGS® i514 primer | 15 μl | 60 μl | |
| TransNGS® i515 primer | 15 μl | 60 μl | |
| TransNGS® i516 primer | 15 μl | 60 μl | |
| TransNGS® i517 primer | 15 μl | 60 μl | |
| TransNGS® i518 primer | 15 μl | 60 μl | |
| TransNGS® i519 primer | 15 μl | 60 μl | |
| TransNGS® i520 primer | 15 μl | 60 μl | |
| TransNGS® i521 primer | 15 μl | 60 μl | |
| TransNGS® i522 primer | 15 μl | 60 μl | |
| TransNGS® i523 primer | 15 μl | 60 μl | |
| TransNGS® i524 primer | 15 μl | 60 μl | |
| i7 Primer | TransNGS® i713 primer | 10 μl | 40 μl |
| TransNGS® i714 primer | 10 μl | 40 μl | |
| TransNGS® i715 primer | 10 μl | 40 μl | |
| TransNGS® i716 primer | 10 μl | 40 μl | |
| TransNGS® i717 primer | 10 μl | 40 μl | |
| TransNGS® i718 primer | 10 μl | 40 μl | |
| TransNGS® i719 primer | 10 μl | 40 μl | |
| TransNGS® i720 primer | 10 μl | 40 μl | |
| TransNGS® i721 primer | 10 μl | 40 μl | |
| TransNGS® i722 primer | 10 μl | 40 μl | |
| TransNGS® i723 primer | 10 μl | 40 μl | |
| TransNGS® i724 primer | 10 μl | 40 μl | |
| TransNGS® i725 primer | 10 μl | 40 μl | |
| TransNGS® i726 primer | 10 μl | 40 μl | |
| TransNGS® i727 primer | 10 μl | 40 μl | |
| TransNGS® i728 primer | 10 μl | 40 μl | |
| TransNGS® i729 primer | 10 μl | 40 μl | |
| TransNGS® i730 primer | 10 μl | 40 μl | |
| TransNGS® i731 primer | 10 μl | 40 μl | |
| TransNGS® i732 primer | 10 μl | 40 μl | |
| TransNGS® i733 primer | 10 μl | 40 μl | |
| TransNGS® i734 primer | 10 μl | 40 μl | |
| TransNGS® i735 primer | 10 μl | 40 μl | |
| TransNGS® i736 primer | 10 μl | 40 μl | |
Universal Primer Information
Sequence of the TransNGS® Universal Primer for Illumina®
5´-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
where -s- indicates the position of the phosphorothioate bond.
TransNGS® i501-i508 Primer
5´-AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
TransNGS® i701-i712 Primer
5´-CAAGCAGAAGACGGCATACGAGAT[i7]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´
-s- indicates phosphorothioate bond.
i5: Index 2, 8 bases;
i7: Index 1, 8 bases.
Index Pooling Guidelines for Single Index Primers
G/T is detected by a green laser and A/C is detected by a red laser when using Illumina sequencer. Color balance is required during the process of sequencing. It is necessary to make sure that both green laser bases and red laser bases are included at each cycle when pooling indexes. Various pooling options can be executed when fewer than 12 indexes are pooled in a lane for sequencing. Some recommended pools are shown below:
- For 2 libraries: Index 6 and 12
- For 3 libraries: Index 4, 6 and 12
- For 4 libraries: Index 4, 6, 8 and 12
Index Pooling Guidelines for Dual Index Primers
G/T is detected by a green laser and A/C is detected by a red laser when using Illumina sequencer. Color balance is required during the process of sequencing. It is necessary to make sure that both green laser bases and red laser bases are included at each cycle when pooling indexes.
If two i5 indexes are used in a lane for sequencing, the following index groups are recommended:
- i501 and i502
- i503 and i504
- i505 and i506
- i507 and i508.
If two i7 indexes are used in a lane for sequencing, the following index groups are recommended:
- i701 and i702
- i703 and i704
- i705 and i706
- i707 and i708
- i709 and i710
- i711 and i712.
Manual & Datasheet
KI201 - TransNGS Single Index Primers Kit for Illumina® (Set 1)
KI211 (coming soon)
KI221 (soon)
Additional information
| Index Set | Set 1, Set 2, Set 3, Dual (96), Dual (384) |
|---|---|
| Format | 48 rx, 96 rx, 192 rx, 384 rx |
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