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TransNGS Index Primers for Illumina® – KI201 KI211 KI221 KI231

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TransNGS Index Primer Kits for Illumina®

Single Index Primer Kits KI201, KI211 and KI221 (Set 1, Set 2 and Set 3 respectively)

TransNGS® Single Index Primer Kits for Illumina® are composed of 3 different sets of primers. Each set of primers contains 12 unique barcoded i7 primers and one NGS Universal Primer for Illumina®.

Dual Index Primer Kit KI231

For multiplex next-generation sequencing library preparations, please use the TransNGS® Dual Index Primer Kit for Illumina®. Dual indexes are ideal for multiplex next-generation sequencing library preparations for Illumina sequencers. The Dual Index Primer kit provides 8 different i5 primers (i501-i508) and 12 different i7 primers (i701-i712). In total, 96 uniquely dual-indexed libraries can be prepared using this kit.

TransNGS™ Index Kits for Illumina® are compatible with other TransNGS series products and with other NGS Library Prep Kits from different vendors.


TransNGS Index Primer Sets for Illumina®

ComponentKI201-01KI201-02KI211-01KI211-02KI221-01KI221-02KI221-01KI221-02
Universal Primer (10 μM)120 μl240 μl120 μl240 μl120 μl240 μl
Index 1 Primer (10 μM)10 μl20 μl
Index 2 Primer (10 μM)10 μl20 μl
Index 3 Primer (10 μM)10 μl20 μl
Index 4 Primer (10 μM)10 μl20 μl
Index 5 Primer (10 μM)10 μl20 μl
Index 6 Primer (10 μM)10 μl20 μl
Index 7 Primer (10 μM)10 μl20 μl
Index 8 Primer (10 μM)10 μl20 μl
Index 9 Primer (10 μM)10 μl20 μl
Index 10 Primer (10 μM)10 μl20 μl
Index 11 Primer (10 μM)10 μl20 μl
Index 12 Primer (10 μM)10 μl20 μl
Index 13 Primer (10 μM)10 μl20 μl
Index 14 Primer (10 μM)10 μl20 μl
Index 15 Primer (10 μM)10 μl20 μl
Index 16 Primer (10 μM)10 μl20 μl
Index 18 Primer (10 μM)10 μl20 μl
Index 19 Primer (10 μM)10 μl20 μl
Index 20 Primer (10 μM)10 μl20 μl
Index 21 Primer (10 μM)10 μl20 μl
Index 22 Primer(10 μM)10 μl20 μl
Index 23 Primer (10 μM)10 μl20 μl
Index 25 Primer (10 μM)10 μl20 μl
Index 27 Primer (10 μM)10 μl20 μl
Index 17 Primer (10 μM)10 μl20 μl
Index 24 Primer (10 μM)10 μl20 μl
Index 26 Primer (10 μM)10 μl20 μl
Index 28 Primer (10 μM)10 μl20 μl
Index 29 Primer (10 μM)10 μl20 μl
Index 30 Primer (10 μM)10 μl20 μl
Index 31 Primer (10 μM)10 μl20 μl
Index 32 Primer (10 μM)10 μl20 μl
Index 33 Primer (10 μM)10 μl20 μl
Index 34 Primer (10 μM)10 μl20 μl
Index 35 Primer (10 μM)10 μl20 μl
Index 36 Primer (10 μM)10 μl20 μl
Index 37 Primer (10 μM)10 μl20 μl
Index 38 Primer (10 μM)10 μl20 μl
Index 39 Primer (10 μM)10 μl20 μl
Index 40 Primer (10 μM)10 μl20 μl
Index 41 Primer (10 μM)10 μl20 μl
Index 42 Primer(10 μM)10 μl20 μl
Index 43 Primer (10 μM)10 μl20 μl
Index 44 Primer (10 μM)10 μl20 μl
Index 45 Primer (10 μM)10 μl20 μl
Index 46 Primer (10 μM)10 μl20 μl
Index 47 Primer (10 μM)10 μl20 μl
Index 48 Primer (10 μM)10 μl20 μl
Dual Index Primer SetKI221-01KI221-02
NGS i501 Primer (10 μM)15μl30 μl
NGS i502 Primer (10 μM)15μl30 μl
NGS i503 Primer (10 μM)15μl30 μl
NGS i504 Primer (10 μM)15μl30 μl
NGS i505 Primer (10 μM)15μl30 μl
NGS i506 Primer (10 μM)15μl30 μl
NGS i507 Primer (10 μM)15μl30 μl
NGS i508 Primer (10 μM)15μl30 μl
NGS i701 Primer (10 μM)10 μl20 μl
NGS i702 Primer (10 μM)10 μl20 μl
NGS i703 Primer (10 μM)10 μl20 μl
NGS i704 Primer (10 μM)10 μl20 μl
NGS i705 Primer (10 μM)10 μl20 μl
NGS i706 Primer (10 μM)10 μl20 μl
NGS i707 Primer (10 μM)10 μl20 μl
NGS i708 Primer (10 μM)10 μl20 μl
NGS i709 Primer (10 μM)10 μl20 μl
NGS i710 Primer (10 μM)10 μl20 μl
NGS i711 Primer (10 μM)10 μl20 μl
NGS i712 Primer (10 μM)10 μl20 μl

Universal Primer Information

Sequence of the TransNGS® Universal Primer for Illumina®
5´-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
where -s- indicates the position of the phosphorothioate bond.

TransNGS® i501-i508 Primer
5´-AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´

TransNGS® i701-i712 Primer
5´-CAAGCAGAAGACGGCATACGAGAT[i7]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´
-s- indicates phosphorothioate bond.
i5: Index 2, 8 bases;
i7: Index 1, 8 bases.


Index Pooling Guidelines for Single Index Primers

G/T is detected by a green laser and A/C is detected by a red laser when using Illumina sequencer. Color balance is required during the process of sequencing. It is necessary to make sure that both green laser bases and red laser bases are included at each cycle when pooling indexes. Various pooling options can be executed when fewer than 12 indexes are pooled in a lane for sequencing. Some recommended pools are shown below:

  • For 2 libraries: Index 6 and 12
  • For 3 libraries: Index 4, 6 and 12
  • For 4 libraries: Index 4, 6, 8 and 12

Index Pooling Guidelines for Dual Index Primers

G/T is detected by a green laser and A/C is detected by a red laser when using Illumina sequencer. Color balance is required during the process of sequencing. It is necessary to make sure that both green laser bases and red laser bases are included at each cycle when pooling indexes.

If two i5 indexes are used in a lane for sequencing, the following index groups are recommended:

  • i501 and i502
  • i503 and i504
  • i505 and i506
  • i507 and i508.

If two i7 indexes are used in a lane for sequencing, the following index groups are recommended:

  • i701 and i702
  • i703 and i704
  • i705 and i706
  • i707 and i708
  • i709 and i710
  • i711 and i712.

Manual & Datasheet

KI201 – TransNGS Single Index Primers Kit for Illumina® (Set 1)

KI211 (coming soon)

KI221 (soon)

KI231 – TransNGS Dual Index Primers Kit for Illumina®

Additional information

Index Set

Set 1, Set 2, Set 3, Dual

Format

48 rx, 96 rx, 192 rx

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