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TransLv Lentivirus Titration qPCR Kit – FV201

695.00$ CAD

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Lentivirus Titration Kit (qPCR)

The TransLvLentivirus Titration Kit provides a simple, rapid, sensitive and efficient method to determine the copy number of integrated proviral sequences in lentivirus transduced cells using qPCR. Lentivirus titer can be calculated from integrated proviral copy number in the genome of the lentivirus transduction susceptible cells (such as HT1080, HEK-293T, etc.) by using the standard curve generated from qPCR.

For Lentivirus precipitation and concentration, please see FV101.

Please ask us for a discount if you wish to purchase both FV101 and FV201.


Benefits of using TransLv Lentivirus qPCR Titration Kit

  • ·Simple operation and high sensitivity

  • Higher accuracy enabled by internal reference gene

  • Compatible with the second and third generation HIV-1 lentiviral packaging vectors

  • Good linear relationship in the range of 103-108 copies/μl


Content

Component
100 reactions
TransLv Lentivirus qPCR Titration SuperMix
1 ml
10×GC enhancer
200 µl
Provirus Gene Standards (P1-P6) (108-103copies/μl)
30 μl each
Reference Gene Standards (R1-R6) (108-103copies/μl)
30 μl each
Provirus Gene Primer Mix (5 μM)
100 µl
Reference Gene Primer Mix (5 μM)
100 µl
Passive Reference Dye(50×)
40 µl
Nuclease-free Water
1ml

Manual and datasheet

FV201 – TransLv Lentivirus qPCR Titration Kit

Additional information

Supplier

Format

100 rx

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