Lentivirus Titration Kit (qPCR)
The TransLv™ Lentivirus Titration Kit provides a simple, rapid, sensitive and efficient method to determine the copy number of integrated proviral sequences in lentivirus transduced cells using qPCR. Lentivirus titer can be calculated from integrated proviral copy number in the genome of the lentivirus transduction susceptible cells (such as HT1080, HEK-293T, etc.) by using the standard curve generated from qPCR.
For Lentivirus precipitation and concentration, please see FV101.
Please ask us for a discount if you wish to purchase both FV101 and FV201.
Benefits of using TransLv Lentivirus qPCR Titration Kit
·Simple operation and high sensitivity
Higher accuracy enabled by internal reference gene
Compatible with the second and third generation HIV-1 lentiviral packaging vectors
Good linear relationship in the range of 103-108 copies/μl
|2×TransLv Lentivirus qPCR Titration SuperMix||1 ml|
|Provirus Gene Standards (P1-P6) (108-103copies/μl)|
|Reference Gene Standards (R1-R6) (108-103copies/μl)|
|Provirus Gene Primer Mix (5 μM)||100 µl|
|Reference Gene Primer Mix (5 μM)||100 µl|
|Passive Reference Dye（50×）||40 µl|
Manual and datasheet
FV201 – TransLv Lentivirus qPCR Titration Kit