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TransB(DE3) Chemically Competent Cell – CD811

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TransB(DE3) Chemically Competent Cell

TransB(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to kanamycin (KanR) and tetracycline (TetR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA). Control Plasmid I (Amp+) is used to detect cell expression function, the size of expressed protein is about 25 kDa.


Features of TransB DE3 cells

  • Transformation efficiency: >107 cfu/μg (pUC19 DNA).
  • KanR and TetR.
  • Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
  • Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

Genotype

F- omp T hsd SB( r B- mB-) gal dcm lac Y1 ahpC (DE3)gor 522::Tn10 trxB (KanR,TetR).

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Manual and Datasheet

CD811 TransB(DE3) Chemically Competent Cell

Additional information

Format

10 x 100 ul

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