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Trans5α ( DH5α ) Competent Cell – CD201

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Description

Trans5α Chemically Competent Cell

Trans5α Chemically Competent Cells are identical to entical to DH5α and are specifically designed for chemical transformation of plasmid DNA (< 6 kb). Trans5 or DH5α offers a transformation efficiency of over 108 cfu/μg DNA (tested with pUC19 plasmid DNA).


Trans5α (DH5α) Characteristics

  • High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
  • Reduced recombination of cloned DNA.
  • Identical to DH5α
  • Blue/white selection.

Trans5α Genotype (DH5α)

F-φ80 lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rk-, mk+) supE44λ- thi -1 gyrA96 relA1 phoA


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Product Manual and Datasheet:

CD201 Trans5a Chemically Competent Cell


Genotype definitions (if applicable to this product)

GenotypeDescription
ara-14Blocks arabinose catabolism
argFOrnithine carbamoyltransferase mutation blocks ability to use arginine
dam/dcmAbolishes endogenous adenine methylation at GATC sequences (dam) or cytosine methylation at CCWGG sequences (dcm). Used to propagate DNA for cleavage with certain restriction enzymes (e.g. Ava II, Bcl I)
DE3Lysogen that encodes T7 RNA polymerase. Used to induce expression in T7-driven expression systems
endAendA Mutation in the non-specific endonuclease Endonuclease I; eliminates non-specific endonuclease activity, resulting in improved plasmid preps
A self-transmissible, low-copy plasmid used for the generation of single-stranded DNA when infected with M13 phage; may contain a resistance marker to allow maintenance and will often carry the lacI and lacZ∆M15 genotypes
galKGalactokinase mutation blocks catabolism of galactose—cells that are galK minus grow in the presence of galactose as the sole carbon source
galUGlucose-1-phosphate uridylyltransferase mutation blocks ability to use galactose—cells that are galU minus can grow on media that contains galactose as the sole carbon source
gyrA96DNA gyrase mutant produces resistance to nalidixic acid
hsdMutations in the system of methylation and restriction that allow E. coli to recognize DNA as foreign. The hsd genotype allows efficient transformation of DNA generated from PCR reactions *hsdR–eliminates restriction of unmethylated EcoK I sites. (1) **hs
lacIEncodes the lac repressor that controls expression from promoters that carry the lac operator; IPTG binds the lac repressor and derepresses the promoter; often used when performing blue/white screening or to control expression of recombinant genes
lacY1Blocks use of lactose via β-D-galactosidase mutant
lacZβ-D-galactosidase gene; mutations yield colorless (vs. blue) colonies in the presence of X-gal
lacZ∆M15Element required for β-galactosidase complementation when plated on X-gal; used in blue/white screening of recombinants; usually carried on the lambdoid prophage φ80 or F´
leuBRequires leucine for growth on minimal media via β-isopropyl malate dehydrogenase mutation
lonlon Deficiency in the Lon ATPase-dependent protease; decreases the degradation of recombinant proteins; all B strains carry this mutation
mcrA, mcrBC,or mrrMutations that allow methylated DNA to not be recognized as foreign; this genotype is necessary when cloning genomic DNA or methylated cDNA
nupGMutation for the transport of nucleosides
ompTIndicates that the E. coli lack an outer membrane protease—reduces degradation of heterologous the strains and recovery of intact recombinant proteins is improved in ompT minus strains
P3A 60-kb low-copy plasmid that carries the ampicillin and tetracycline resistance genes with amber mutations; used predominantly for selection of supF-containing plasmids; carries the kanamycin resistance gene for selection
pLyspLys Plasmid that encodes T7 lysozyme; used to reduce basal expression in T7-driven expression systems by inhibiting basal levels of T7 RNA polymerase
proABproAB Requires proline for growth on minimal media
recAMutation in a gene responsible for general recombination of DNA; particularly desirable when cloning genes with direct repeats
relARNA is synthesized in absence of protein synthesis (relaxed phenotype) relA locus regulates the coupling between transcription and translation. In the wild type, limiting amino acid concentrations results in the shutdown of RNA synthesis (also known as th
rpsLConfers resistance to streptomycin (this makes a mutant ribosomal protein, small subunit, the target of the drug)
supE,FtRNA glutamine suppressor of amber (supE)(UAG) or tyrosine (supF)
thi-1Requires thiamine for growth on minimal media
Tn10Confers tetracycline resistance via a transposon
tonAConfers resistance to the lytic bacteriophage T1, T5 and f80
traD, D36Prevents transfer of F’ episome via transfer factor mutation
tsxConfers resistance to phage T6 and colicin K
xyl-5Blocks catabolism of xylose

Additional information

Format

10 x 100 ul, 20 x 100 ul, BULK 100 x 100 ul (10 ml)

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