Tar DNA binding protein 43 Antibody – MCA-3H8

Encor Mouse Monoclonal to Tar DNA Binding Protein 43 (TARBDP)

TDP43 was originally identified as a protein which binds to the “transactivation response” (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (1). UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain a two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus.
The protein is frequently referred to by the acronym TDP43 corresponds to “TAR DNA binding protein of molecular weight 43 kDa”, although the Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name is TARDBP. Northern blots showed that the protein is ubiquitous in tissue expression.

Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (ALS, 2). The protein is present in these inclusions in a partially degraded, hyperphosphorylated and ubiquitinated form. For a recent review of the role in TDP43 in various neurological disorders see reference 3.

This antibody has been used in a recent peer reviewed publication, showing that it works well on western blots and on histological sections (4). Further images of histological staining using this antibody showing clears staining of ALS related inclusion bodies is shown here.

MCA-3H8 is a mouse IgG1 class antibody and was raised against full length recombinant human TDP43 expressed in and purified from E. coli. MCA-3H8 is known to react with TDP43 from human, cow, pig, mouse, rat and other mammals. Since the TDP43 protein sequence is highly conserved across species barriers, it is likely that the antibody is effective on many other species also.

HGNC name(s) : TARBDP

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Tar DNA binding protein 43 3H8

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Conjugation : none

Immunogen : Recombinant full length human

Mass of detected protein : 43 kDa

Uniprot ID : Q13148

KGNC name : TARBDP

RRID # : AB_2572387

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:5 000. ICC/IF and IHC: 1:1 000

Datasheet

Link to on EnCor’s website

References :

1. Ou SH, Wu F, Harrich D, García-Martínez LF and Gaynor RB. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J Virol. 69:3584-96 (1995).

2. Neumann, M., Sampathu, D. M., Kwong, L. K., Truax, A. C., Micsenyi, M. C., Chou, T. T., Bruce, J., Schuck, T., Grossman, M., Clark, C. M., McCluskey, L. F., Miller, B. L., Masliah, E., Mackenzie, I. R., Feldman, H., Feiden, W., Kretzschmar, H. A., Trojanowski, J. Q., Lee, V. M.-Y. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 314:130-133 (2006).

3. Forman MS, Trojanowski JQ and Lee VM-Y. TDP-43: a novel neurodegenerative proteinopathy. Current Opinions in Neurobiology 17:548-55 (2007).

4. Wilson AC, Dugger BN, Dickson DW and Wang DS. TDP-43 in aging and Alzheimer’s disease – a review. Int J Clin Exp Pathol. 4:147-55 (2011).