T4 DNA Ligase – FL101
$56.00 – $96.00 CAD
T4 DNA Ligase
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-strand nucleic acids. This enzyme requires ATP as a cofactor.
Source
E.coli strain carrying T4 DNA ligase gene
Unit Definition
One unit is the amount of enzyme required to give 50% ligation of Hind III fragments of λ DNA (5 ́ DNA termini concentration of 0.12 μM, 200 μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 Ligase Buffer.
Quality Control
Functional absence of endonucleases and exonucleases activities
Applications for T4 DNA Ligase
- Cloning blunt-end or cohesive-end fragments.
- Ligation of synthetic linkers or adaptors.
Storage at -20 °C for one year
Datasheet and manual
Additional information
| Format | 5 x 20 000 u, 10 000 u, 20 000 u |
|---|---|
| Supplier |
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Bin –
Tried this T4 ligase as an alternative to the T4 Ligase that we nornally use. Ligation was a success!
srcivicbio (verified owner) –
Thank you Bin for your feedback. Glad you enjoyed Transgen’s T4 DNA Ligase. I’ll email your lab manager a promo code for a 10% discount. All the best