Select Page

T4 DNA Ligase – FL101

Rated 4.00 out of 5 based on 1 customer rating
(1 customer review)

Product Description

T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-strand nucleic acids. This enzyme requires ATP as a cofactor.


E.coli strain carrying T4 DNA ligase gene

Unit Definition

One unit is the amount of enzyme required to give 50% ligation of Hind III fragments of λ DNA (5 ́ DNA termini concentration of 0.12 μM, 200 μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 Ligase Buffer.

Quality Control

Functional absence of endonucleases and exonucleases activities

Applications for T4 DNA Ligase

  • Cloning blunt-end or cohesive-end fragments.
  • Ligation of synthetic linkers or adaptors.


TGFL101 content

Storage at -20 °C for one year

Datasheet and manual

FL101 T4 DNA Ligase

Additional information


5 x 20 000 u, 10 000 u, 20 000 u

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

DNA Polymerase Comparison Chart

Commercial DNA Polymerase Comparison Chart DNA Polymerase Comparison of different commercially available enzymes with their genral specifications and pricing in Canadian dollars.

PCR Purification Kit Comparison – DNA Clean Up

Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...

Product Reviews for T4 DNA Ligase – FL101

1 review for T4 DNA Ligase – FL101

  1. Rated 4 out of 5


    Tried this T4 ligase as an alternative to the T4 Ligase that we nornally use. Ligation was a success!

    • srcivicbio (verified owner)

      Thank you Bin for your feedback. Glad you enjoyed Transgen’s T4 DNA Ligase. I’ll email your lab manager a promo code for a 10% discount. All the best

Add a review

Your email address will not be published. Required fields are marked *

Order Today!

50.00$295.00$ CAD


Cashback Reward : Earn up to 29$!

SKU: FL101 Category: Tags: , Brand: