Splicing factor SF3B4 Antibody – MCA-3A1

Encor Mouse Monoclonal to Splicing factor SF3B4

Splicing factor SF3B4, also known as SAP49, is a ubiquitously expressed splicing factor found in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells. It was originally identified by as the protein most efficiently UV cross-linked to the A, B and C spliceosomal complexes (1).
The protein contains two of the highly conserved RRM type RNA recognition motifs, each corresponding to a small ~70 amino acid structure, consisting of 4 beta strands and two alpha-helices. Proteins containing these proteins are believed to have a role in the regulation of mRNA splicing. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49kDa. This protein is known as splicing factor 3b, subunit 4, 49kDa SAP49, spliceosome-associated protein (U2 snRNP), Hsh49 and MGC108282. Antibodies to this protein are good markers of nuclei. The HGNC name for this protein is SF3B4.

HGNC name(s) : SF3B4

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Splicing factor SF3B4 3A1

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG2b

Conjugation : none

Immunogen : Recombinant full length human

Mass of detected protein : 49 kDa

Uniprot ID : Q15427

KGNC name : SF3B4

RRID # : AB_2572386

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:1 000. ICC/IF and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website

References :

Champion-Arnaud P, Reed R. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes Dev. 8:1974-83 (1994).