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RNase H – LH101

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SKU: LH101 Category: Tag: Brand:

Ribonuclease H

Ribonuclease H (RNase H) specilically degrades the RNA strand from RNA:DNA hybrids. It does not hydrolyse the phosphodiester
bond from single-stranded or double stranded DNA or RNA. RNase H is purified from E.Coli expressing the recombinant rnhA gene. The recombinant protein has a molecular mass of 17 kDa and can be heat-inactivated by incubation at 65°C for 20 min.


Applications for RNase H

  • Removal of RNA after first-strand synthesis by reverse transcription to allow for second strand synthesis reaction.
  • Identification of RNA:DNA hybrids

Contents

RNase H : 100 u / 5 x 100 u

10x RNase H Buffer : 1 ml / 5 x 1 ml


Manual and datasheet

RNase H

Additional information

Format

5 x 100 u, 100 u

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