RNase H – LH101
33.00$ – 132.00$ CAD
0 in stock (expect 7-14 days for delivery)
Ribonuclease H (RNase H) specilically degrades the RNA strand from RNA:DNA hybrids. It does not hydrolyse the phosphodiester
bond from single-stranded or double stranded DNA or RNA. RNase H is purified from E.Coli expressing the recombinant rnhA gene. The recombinant protein has a molecular mass of 17 kDa and can be heat-inactivated by incubation at 65°C for 20 min.
Applications for RNase H
- Removal of RNA after first-strand synthesis by reverse transcription to allow for second strand synthesis reaction.
- Identification of RNA:DNA hybrids
RNase H : 100 u / 5 x 100 u
10x RNase H Buffer : 1 ml / 5 x 1 ml
Manual and datasheet
5 x 100 u, 100 u