Rat Smad3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit
The Smad3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Smad3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Smad3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Smad3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Smad3. You can calculate the concentration of Smad3 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Mothers Against Decapentaplegic Homolog 3
Abbreviation : Smad3
Uniprot ID : P84025
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 0.094 ng/ml
Detection Range : 0.156 – 10 ng/ml
Additional information
Format | 96 rx |
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