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Rat PRF1 (Perforin 1) ELISA Kit – E-EL-R0721

96 rx

797.00$ CAD

0 in stock (expect 7-14 days for delivery)

0 in stock (expect 7-14 days for delivery)

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SKU: E-EL-R0721 Category: Tags: , Brand:

Rat PRF1 (Perforin 1) ELISA Kit

The PRF1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for PRF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PRF1. You can calculate the concentration of PRF1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Perforin 1

Abbreviation : PRF1

Uniprot ID : P35763

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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96 rx

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