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Rat PPAR-γ (Peroxisome Proliferator-Activated Receptor γ) ELISA Kit – E-EL-R0724

96 rx

$869.00 CAD

0 in stock (expect 7-21 days for delivery)

0 in stock (expect 7-21 days for delivery)

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SKU: E-EL-R0724 Category: Tags: , Brand:

Rat PPAR-γ (Peroxisome Proliferator-Activated Receptor γ) ELISA Kit

The PPAR-γ ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PPAR-γ. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PPAR-γ. You can calculate the concentration of PPAR-γ in the samples by comparing the OD of the samples to the standard curve.


Detected Target name: Rat Peroxisome Proliferator-Activated Receptor γ


Abbreviation : PPAR-γ


Uniprot ID : O88275


Format : 96 rx


Reactivity : Rat


ELISA configuration : Sandwich


Sensitivity : 0.094 ng/ml


Detection Range : 0.156 – 10 ng/ml


Manual & Datasheet


More information about this Rat PPAR-γ (Peroxisome Proliferator-Activated Receptor γ) ELISA Kit from Elabscience


Additional information

Format

96 rx

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