Rat OPN (Osteopontin) ELISA Kit – E-EL-R0702

Rat OPN (Osteopontin) ELISA Kit

The OPN ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OPN. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for OPN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of OPN. You can calculate the concentration of OPN in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Osteopontin

Abbreviation : OPN

Uniprot ID : P08721

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Rat OPN (Osteopontin) ELISA Kit from Elabscience