Rat MAPK14 (Mitogen Activated Protein Kinase 14) ELISA Kit – E-EL-R0711

Rat MAPK14 (Mitogen Activated Protein Kinase 14) ELISA Kit

The MAPK14 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MAPK14. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for MAPK14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MAPK14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MAPK14. You can calculate the concentration of MAPK14 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Mitogen Activated Protein Kinase 14

Abbreviation : MAPK14

Uniprot ID : P70618

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.75 ng/ml

Detection Range : 1.25 – 80 ng/ml

Manual & Datasheet

More information about this Rat MAPK14 (Mitogen Activated Protein Kinase 14) ELISA Kit from Elabscience