Rat CA125 (Carbohydrate Antigen 125) ELISA Kit – E-EL-R0707

Rat CA125 (Carbohydrate Antigen 125) ELISA Kit

The CA125 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Ovarian cancer marker/CA125. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Ovarian cancer marker/CA125 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Ovarian cancer marker/CA125, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Ovarian cancer marker/CA125. You can calculate the concentration of Ovarian cancer marker/CA125 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Carbohydrate Antigen 125

Abbreviation : CA125

Uniprot ID : D3ZM49

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 4.688U/mL

Detection Range : 7.813 – 500U/mL

Manual & Datasheet

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