Rat 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit – E-EL-R1001

Rat 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit

The 15-LO ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat 15-LO. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat 15-LO. You can calculate the concentration of Rat 15-LO in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Arachidonate 15-Lipoxygenase

Abbreviation : 15-LO

Uniprot ID : Q02759

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Rat 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit from Elabscience