Rat 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit
The 12-LO ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat 12-LO. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat 12-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat 12-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat 12-LO. You can calculate the concentration of Rat 12-LO in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Arachidonate 12-Lipoxygenase
Abbreviation : 12-LO
Uniprot ID : F1LQ70
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 0.938 ng/ml
Detection Range : 1.563 – 100 ng/ml
More information about this Rat 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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