PGI2 (Prostacyclin) ELISA Kit
The PGI2 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with PGI2. During the reaction, Universal PGI2 in the sample or standard competes with a fixed amount of PGI2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGI2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: PGI2
Abbreviation : prostaglandin I2
Format : 96 rx
Reactivity : Universal (All Species expexted)
ELISA configuration : Competitive
Sensitivity : 18,75
Detection Range : 31.25-2000 pg/mL
More information about this PGI2 (Prostacyclin) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
---|---|
Reactivity | Universal |
Reviews