Pg (Progesterone) ELISA Kit – E-EL-0090

Pg (Progesterone) ELISA Kit

The This ELISA kit uses Competitive-ELISA as themethod. The microtiter plate provided in this kit has been pre-coated with Goat Anti-Rabbit IgG, make solid-phase secondary antibody. And then add sample,horseradish peroxidase-labeled Progesterone and anti- Progesterone antibody, soas to form a coated secondary antibody – anti- Progesterone antibody -HRP-labeled Progesterone complex. The amount of bound labeled Progesterone isinversely proportional to the amount of Progesterone in the sample. TMB substrate solution is added to each well after wash the microtiter plate, TMB turn to blue catalyst with horseradish peroxidase (HRP). The enzyme-substratereaction is terminated by the addition of a sulphuric acid solution and thecolor change is measured spectrophotometrically at a wavelength of 450 nm ± 2nm. The concentration of Pg in the samples is then determined by comparing the OD of the samples to the standard curve.<

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Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,281

Detection Range : 0.469-30 ng/mL

More information about this Pg (Progesterone) ELISA Kit from Elabscience