Encor Rabbit polyclonal to Neurofilament Medium, NF-M
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed of a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, α-internexin, nestin and, in some cases, vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. This antibody was raised against the C-terminal region of NF-M, a region highly conserved between NF-M molecules in different species. Accordingly the antibody recognizes all mammalian and avian NF-M molecules. The name NF-M refers to the fact that this is the “middle” or “medium” protein of neurofilament triplet, the other two proteins being “low” or “light” (NF-L) and “high” or “heavy” (NF-H). NF-M is sometimes also referred to as NEF3 or Neurofilament 3. The HGNC name for this protein is NEFM.
Antibody was raised in rabbit which was injected with a recombinant fusion protein containing the extreme C-terminal segment of rat NF-M. The antibody is similar to that described in reference 1 below, and has been marketed by many companies for many years.
HGNC name(s) : NEFM
Host : Rabbit
Clonality : Polyclonal
ID : EnCor Biotechnology Neurofilament NF-M NF-M
Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
Isotype : IgG
Conjugation : none
Immunogen : Recombinant fusion protein with extreme C-term rat NF-M
Mass of detected protein : 145 kDa by SDS-PAGE
Uniprot ID : P07197
KGNC name : NEFM
RRID # : AB_2572366
Purification : Serum
Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Validated applications : WB | IF/ICC | IHC
Suggested Dilutions:
WB: 1:1 000-5 000. IF/ICC and IHC: 1:1 000-1:2500. ABC: 1:5 000.
References :
1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.
2. Wang, Y., Zhang, J., Mori, S. and Nathans. J. Axonal Growth and Guidance Defects in Frizzled3 Knock-Out Mice: A Comparison of Diffusion Tensor Magnetic Resonance Imaging, Neurofilament Staining, and Genetically Directed Cell Labeling. J. Neurosci. 26:355-364 2006.
Additional information
Format | 50 ul, 100 ul, 500 ul |
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Supplier | |
Host | Rabbit |
Clonality | Polyclonal |
Conjugation | None |
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