Neurofilament NF-L Antibody – RPCA-NF-L

Encor Rabbit polyclonal to Neurofilament Light, NF-L

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease and Alzheimer’s disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (1).The HGNC name for this protein is NEFL.

HGNC name(s) : NEFL

Host : Rabbit

Clonality : Polyclonal

ID : EnCor Biotechnology Neurofilament NF-L NF-L

Reactivity : Human | Rat | Mouse

Isotype : IgG

Conjugation : none

Immunogen : Purified porci ne

Mass of detected protein : 68 kDa by SDS-PAGE

Uniprot ID : P07196

KGNC name : NEFL

RRID # : AB_2572364

Purification : Serum

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1: 10 000-1:15 000. IF/ICC: 1:5 000.

Datasheet

Link to on EnCor’s website

References :

1. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46, (2000).

2. Delacourte A, Filliatreau G, Boutteau F, Biserte G and Schrevel J. Study of the 10-nm-filament fraction isolated during the standard microtubule preparation. Biochem J. 191:543-6. (1980).