Neurofilament NF-L Antibody – MCA-7D1

Encor Mouse Monoclonal to Neurofilament Light chain, NF-L. A marker of neuronal cells and processes.

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament lite or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease and Alzheimer’s disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (1).

MCA-7D1 was raised against a preparation of pig neurofilament NF-H and NF-L. Screening was done by ELISA on the immunogen followed by immunofluorescence microscopy. Clones which revealed strong staining were further characterized biochemically. MCA-7D1 is a IgG2b class antibody. It reacts with NF-L from human, cow, pig, mouse, rat and all other mammals. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.

HGNC name(s) : NEFL

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Neurofilament NF-L 7D1

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG2b

Conjugation : none

Immunogen : Purified porci ne

Mass of detected protein : 68 kDa by SDS-PAGE

Uniprot ID : P07196

KGNC name : NEFL

RRID # : AB_2572363

Purification : Ascites

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:5 000-1:10 000. IF/ICC and IHC: 1:100-1:500.

Datasheet

Link to on EnCor’s website

References :

1. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46, 2000.