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Neurofilament NF-L Antibody – MCA-7D1

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Encor Mouse Monoclonal to Neurofilament Light chain, NF-L. A marker of neuronal cells and processes. 

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament lite or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease and Alzheimer’s disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (1).

MCA-7D1 was raised against a preparation of pig neurofilament NF-H and NF-L. Screening was done by ELISA on the immunogen followed by immunofluorescence microscopy. Clones which revealed strong staining were further characterized biochemically. MCA-7D1 is a IgG2b class antibody. It reacts with NF-L from human, cow, pig, mouse, rat and all other mammals. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.


HGNC name(s) : NEFL


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Neurofilament NF-L 7D1


Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse


Isotype : IgG2b


Conjugation : none


Immunogen : Purified porci ne


Mass of detected protein : 68 kDa by SDS-PAGE


Uniprot ID : P07196


KGNC name : NEFL


RRID # : AB_2572363


Purification : Ascites


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:5 000-1:10 000. IF/ICC and IHC: 1:100-1:500.


Datasheet


Link to on EnCor’s website 


References :

1. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46, 2000.


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Mouse

Clonality

Monoclonal

Conjugation

None

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