Neurofilament NF-H Antibody – RPCA-NF-H

Encor Rabbit Polyclonal to Neurofilament NF-H

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed as a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments.
The HGNC name for this protein is NEFH.

HGNC name(s) : NEFH

Host : Rabbit

Clonality : Polyclonal

ID : EnCor Biotechnology Neurofilament NF-H NF-H

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG

Conjugation : none

Immunogen : Purified bovine

Mass of detected protein : 200 kDa

Uniprot ID : P12036

KGNC name : NEFH

RRID # : AB_2572360

Purification : Serum

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000-25 000. ICC/IF and IHC: 1:1 000-5 000.

Datasheet

Link to on EnCor’s website

References :

1. Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK and Howland DR. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 336:1268-1277 (2005).