Neurofilament NF-H Antibody – MCA-AH1

Encor Mouse Monoclonal to Neurofilament NF-H

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries.
Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig’s disease) and Alzheimer’s disease.

MCA-AH1 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with MCA-AH1 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH.

MCA-AH1 is a mouse monoclonal antibody raised against a preparation of native NF-H purified from bovine spinal cord. MCA-AH1 is an IgG1 class antibody with a k light chain. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

The characterization of this antibody has been published (1).

HGNC name(s) : NEFH

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Neurofilament NF-H AH1

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Conjugation : none

Immunogen : Purified bovine

Mass of detected protein : 200 kDa

Uniprot ID : P12036

KGNC name : NEFH

RRID # : AB_2572357

Purification : Ascites

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000. ICC/IF and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website

References :

1. Boylan K, Yang C, Crook J, Overstreet K, Heckman M, Wang Y, et al. Immunoreactivity of the phosphorylated axonal neurofilament H subunit (pNF-H) in blood of ALS model rodents and ALS patients: evaluation of blood pNF-H as a potential ALS biomarker. J. Neurochem. 111:1182-1191 (2009).