Myelin Basic Protein, MBP Antibody – MCA-7G7
- Clonality : Mouse Monoclonal
- Applications : WB | IF/ICC | IHC
- Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
310.00$ – 1,660.00$ CAD
0 in stock (expect 7-14 days for delivery)
Encor Mouse Monoclonal to Myelin Basic Protein, MBP
Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (1). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (2). In this case the proteins are referred to as Golli-MBP proteins and result from the use of 3 exons not utilized in the nervous system, resulting in proteins with unique N-terminal regions.
In the central nervous system there are four different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 20.5, 18.5, and 17.2 kDa in humans. The single gene of rodents also produces 4 different proteins, but of slightly different sizes, 21.5, 18.5, 17 and 14 kDa. Sequence alignments of both human and rodent isotypes can be downloaded from here. We have found that our chicken antibody stains all four transcripts on blots with equivalent intensity. We also market two good mouse monoclonal antibodies to MBP, MCA-7G7 and MCA-7D2. The HGNC name for this protein is MBP.
Antibody was raised in chicken against purified myelin basic protein isolated from cow nerve. This antibody is an IgY preparation, with total protein content about 30 mg/mL.
HGNC name(s) : MBP
Host : Mouse
Clonality : Monoclonal
ID : EnCor Biotechnology Myelin Basic Protein, MBP 7G7
Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
Isotype : IgG1
Conjugation : none
Immunogen : Purified bovine
Mass of detected protein : 14, 17, 18.5, and 21.5 kDa in rodent, 17.2, 18.5, 20.5 and 21.5 in human
Uniprot ID : P02686
KGNC name : MBP
RRID # : AB_2572353
Purification : Ascites
Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Validated applications : WB | IF/ICC | IHC
WB:1:5 000 -1:10 000. IF/ICC and IHC: 1:1 000.
1. Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. Basic A1 protein of the myelin membrane. The complete amino acid sequence. J. Biol. Chem. 246:5770-5784 (1971).
2. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861 (2002).
50 ul, 100 ul, 500 ul