Select Page

Myelin Basic Protein, MBP Antibody – MCA-7G7

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

299.00$1,597.00$ CAD

Clear

Cashback Reward : Earn up to 159$!


Encor Mouse Monoclonal to Myelin Basic Protein, MBP 

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (1). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (2). In this case the proteins are referred to as Golli-MBP proteins and result from the use of 3 exons not utilized in the nervous system, resulting in proteins with unique N-terminal regions.
In the central nervous system there are four different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 20.5, 18.5, and 17.2 kDa in humans. The single gene of rodents also produces 4 different proteins, but of slightly different sizes, 21.5, 18.5, 17 and 14 kDa. Sequence alignments of both human and rodent isotypes can be downloaded from here. We have found that our chicken antibody stains all four transcripts on blots with equivalent intensity. We also market two good mouse monoclonal antibodies to MBP, MCA-7G7 and MCA-7D2. The HGNC name for this protein is MBP.

Antibody was raised in chicken against purified myelin basic protein isolated from cow nerve. This antibody is an IgY preparation, with total protein content about 30 mg/mL.


HGNC name(s) : MBP


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Myelin Basic Protein, MBP 7G7


Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse


Isotype : IgG1


Conjugation : none


Immunogen : Purified bovine


Mass of detected protein : 14, 17, 18.5, and 21.5 kDa in rodent, 17.2, 18.5, 20.5 and 21.5 in human


Uniprot ID : P02686


KGNC name : MBP


RRID # : AB_2572353


Purification : Ascites


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB:1:5 000 -1:10 000. IF/ICC and IHC: 1:1 000.


Datasheet


Link to on EnCor’s website 


References :

1. Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. Basic A1 protein of the myelin membrane. The complete amino acid sequence. J. Biol. Chem. 246:5770-5784 (1971).

2. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861 (2002).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier
Host
Clonality
Reactivity
Validated Applications
Conjugation
Isotype

Related Products

DNA Polymerase Comparison Chart

DNA Polymerase Comparison Chart

  Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.      

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

Product Reviews for Myelin Basic Protein, MBP Antibody – MCA-7G7

Reviews

There are no reviews yet.

Be the first to review “Myelin Basic Protein, MBP Antibody – MCA-7G7”

Your email address will not be published. Required fields are marked *

Order Today!

299.00$1,597.00$ CAD

Clear

Cashback Reward : Earn up to 159$!