TransSafe Mycoplasma Elimination Reagent (TransMyco-1+2) – FM401

TransSafe® Mycoplasma Elimination Reagent (TransMyco 1+2) – FM401

TransSafe® Mycoplasma Elimination Reagent (TransMyco 1+2) contains two different and effective anti-mycoplasma components. TransMyco-1 is a macrolide antibiotic, and TransMyco-2 is a tetracycline derivative. Both antibiotics effectively interfere with mycoplasma protein synthesis to eliminate mycoplasma species from contaminated cell cultures. The joint use of these two reagents allows to eliminate the contamination by mycoplasma fwith minimal cytotoxicity to the cell culture.

Benefits of using TransSafe® Mycoplasma Elimination Reagent (TransMyco 1+2)

• Healthy cell cultures:
  • eliminates mycoplasma effectively.
  • low cytotoxicity
• High Potency : low working concentration.
• Cell Compatibility: Suitable for a wide range of cell types.
• Easy to use : Ready to use. Add to the culture medium directly.

Mycoplasma decontamination using TransMyco 1+2

Important Notes

• TransSafe Mycoplasma Elimination Reagent (TransMyco-3) has been shown to be a good way to remove the mycoplsma contamination from a variety of cell lines including HepG2, HEK-293T, HEK-293, HeLa, N2a, A549, MCF-7, CHO, K562, NIH/3T3, Sp2/0, BHK-21, Vero, U937, SH-SY5Y and more. It also has a potent effect on primary cell cultures.
• Prior to use, ensure that the bottle cap is sealed. Thaw to room temperature. Mix gently and use 70% ethanol to wipe the bottle surface, and then put on the clean bench.
• This product is stored at -20℃. Avoid repeated freezing and thawing. It should be added fresh culture medium. It can be stored at 4℃ within a week for the short term storage.
• For cell lines with fast proliferation, in order to reduce the number of passage, it is recommended to reduce the density of cells or appropriate to reduce the concentration of serum in medium.
• If the cells are very sensitive to TransMyco-1 or 2, its concentration can be reduced.
• The combination of TransMyco-1 + TransMyco-2, and TransMyco-3 alone, are two different ways for the treatment of mycoplasma contamination. For mycoplasma contamination of different types, the effect may be slightly different. In order to ensure the treatment effect and improve the efficiency. One may group the cells in two, treat one with TransMyco-1+2, the other with TransMyco-3. And then select the method with better result for subsequent experiments.
• If there is contamination of mycoplasma in the environment or the reagents used for cell culture, cells are easy to be re-contaminated with mycoplasma. We recommend that the mycoplasma prevention reagent, such as TransSafe Mycoplasma Prevention Reagent – FM501 – should be added into culture medium to prevent mycoplasma contamination. In addition, monthly testing for mycoplasma contamination is recommended with either the Mycoplasma Detection PCR Kit or Mycoplasma Detection Luciferase Kit.

Contents

Manual and datasheet:

TransSafe Mycoplasma Elimination Reagent (TransMyco-1+2) -2017-02