Mouse sFRP4 Protein (His tag) – PKSM040882

Recombinant Mouse sFRP4 Protein (His tag)

SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain . Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.

Synonym(s) : Sfrp4

Species(s) : Mouse

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the mouse sFRP4 (NP_057896.1) (Met 1-Ser 351) was expressed with a C-terminal polyhistidine tag.

Predicted N-terminal amino acid : Ala 22

Molecular weight/mass : The recombinant mouse sFRP4 consists of 341 amino acids after removal of the signal peptide and has a predicted molecular mass of 39.4 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.

Protein Construction : A DNA sequence encoding the mouse sFRP4 (NP_057896.1) (Met 1-Ser 351) was expressed with a C-terminal polyhistidine tag.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet