Mouse ACE (Angiotensin I Converting Enzyme) ELISA Kit – E-EL-M0091

Mouse ACE (Angiotensin I Converting Enzyme) ELISA Kit

The Mouse ACE ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACE. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ACE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACE. You can calculate the concentration of ACE in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Angiotensin I Converting Enzyme

Abbreviation : ACE

Uniprot ID : P09470

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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