Mouse ABCA1 (ATP Binding Cassette Transporter A1) ELISA Kit – E-EL-M0164

Mouse ABCA1 (ATP Binding Cassette Transporter A1) ELISA Kit

The Mouse ABCA1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ABCA1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ABCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ABCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ABCA1. You can calculate the concentration of ABCA1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse ATP Binding Cassette Transporter A1

Abbreviation : ABCA1

Uniprot ID : P41233

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Mouse ABCA1 (ATP Binding Cassette Transporter A1) ELISA Kit from Elabscience