Monkey D2D (D-Dimer) ELISA Kit – E-EL-MK0385

Monkey D2D (D-Dimer) ELISA Kit

The D2D ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to D2D. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for D2D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain D2D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of D2D. You can calculate the concentration of D2D in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: D-Dimer

Abbreviation : D2D

Uniprot ID :

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Competitive

Sensitivity : 37.5 ng/ml

Detection Range : 62.5 – 4000 ng/ml

Manual & Datasheet

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