Monkey BALP (Bone Alkaline Phosphatase) ELISA Kit – E-EL-MK1151

Monkey BALP (Bone Alkaline Phosphatase) ELISA Kit

The BALP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BALP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BALP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BALP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BALP. You can calculate the concentration of BALP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Bone Alkaline Phosphatase

Abbreviation : BALP

Uniprot ID :

Format : 96-well plate

Reactivity : Monkey

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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