Microtubule Associated Protein 2, MAP2C/D Antibody – MCA-2C4

Encor Mouse Monoclonal to Microtubule Associated Protein 2, MAP2C/D

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2A and MAP2B.
A third lower molecular weight form, usually called MAP2C and MAP2D, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All four MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (a.k.a. MAP-tau or tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers of neuronal cells, their perikarya and neuronal dendrites. MCA-2C4 was raised against recombinant human MAP2D and also binds to recombinant human MAP2C. The antibody epitope is therefore in the core of the MAP2 molecule and so this antibody will bind all known MAP2 isoforms as shown in the blot. MAP2C and MAP2D are expressed earlier in development than the MAP2a and MAP2b isoforms, so that this antibody is a more useful marker of neuronal development. In contrast MAP-τ (MAP-tau) is found predominantly but not exclusively in neuronal axons. The HGNC name for this protein is MAP2.

HGNC name(s) : MAP2

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Microtubule Associated Protein 2, MAP2C/D 2C4

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Conjugation : none

Immunogen : Full length recombinant human

Mass of detected protein : MAP2A/B ~2*0 kDa, MAP2C/D ~70kDa by SDS-PAGE

Uniprot ID : P11137

KGNC name : MAP2

RRID # : AB_2572215

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:5 000-1:10 000. ICC/IF and IHC: 1:5 000-10 000.

Datasheet

Link to on EnCor’s website

References :

1. Dehmelt L, Halpain S. The MAP2/Tau family of microtubule-associated proteins. Genome Biol. 6:204 (2004)

2. Vallee R. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). J. Cell Biol. 92:435-442 (1992)