MARCKS Antibody – RPCA-MARCKS

Encor Rabbit Polyclonal to MARCKS (Myristoylated alanine rich C Kinase substrate)

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered by as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells (1). The MARCKS protein runs at about 80 kDa on SDS-PAGE gels, although the real molecular is much lower, at about 31.5 kDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less to SDS than the average protein, and so migrate in PAGE more slowly than the normal protein. MARCKS is a major protein of the brain, and is concentrated in the synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind to both actin and calmodulin in vitro (2). MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties (3). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (4).
The HGNC name for this protein is MARCKS.

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HGNC name(s) : MARCKS

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Host : Rabbit

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Clonality : Polyclonal

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ID : EnCor Biotechnology MARCKS MARCKS

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Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Isotype : IgG

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Conjugation : none

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Immunogen : Recombinant full length human

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Mass of detected protein : 80 kDa

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Uniprot ID : P29966

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KGNC name : MARCKS

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RRID # : AB_2250333

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Purification : Serum

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Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

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Validated applications : WB | IF/ICC | IHC

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Suggested Dilutions:

WB: 1:10 000-1:20 000. IF/ICC and IHC: 1:500-1:1 000. ABC: 1:5 000.

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Datasheet

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Link to on EnCor’s website

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References :

1. Hirai, M. and Shimizu N. Purification of two distinct proteins of approximate Mr 80,000 from human epithelial cells and identification as proper substrates for protein kinase C. Biochem J. 270:583-9 (1990).

2. Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622 (1992)

3. Blackshear PJ. The MARCKS family of cellular protein kinase C substrates. J. Biol. Chem. 268: 1501-1504 (1993).

4. Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92, 944-948 (1995)

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