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Lamin A/C Antibody – CPCA-LaminAC

  • Clonality : Chicken Polyclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Encor Chicken Polyclonal to Lamin A/C 

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane.
Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently, it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other.

This is the case with this polyclonal, which was raised against full length recombinant human Lamin A. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of “maturation promoting factor“, the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina.

Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3). The HGNC name for this protein is LMNA.


HGNC name(s) : LMNA


Host : Chicken


Clonality : Polyclonal


ID : EnCor Biotechnology Lamin A/C LaminAC


Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse


Isotype : IgY


Conjugation : none


Immunogen : Recombinant full length human


Mass of detected protein : 65 and 74 kDa


Uniprot ID : P02545


KGNC name : LMNA


RRID # : AB_2572338


Purification : Concentrated IgY preparation


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:2 000. IF/ICC and IHC: 1:1 000 .


Datasheet


Link to on EnCor’s website 


References :

1. Fisher, D. Z., Chaudhary, N., Blobel, G. cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins. Proc. Nat. Acad. Sci. 83: 6450-6454 (1986).

2. McKeon, F. D., Kirschner, M. W., Caput, D. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins. Nature 319: 463-468 (1986).

3. Liu, B. and Zhou, Z. Lamin A/C, laminopathies and premature ageing. Histol. Histopathol. 23: 747-763 (2006).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Chicken

Clonality

Polyclonal

Reactivity

Chicken, Cow, Horse, Human, Mouse, Pig, Rat

Validated Applications

WB, IHC, IF/ICC

Conjugation

None

Isotype

IgY

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299.00$1,597.00$ CAD

Clear

Cashback Reward : Earn up to 159$!