KLD Mix for Back-to-Back Site-Directed Mutagenesis
110.00$ – 425.00$ CAD
KLD Mix Site-Directed Mutagenesis
The KLD Mix for Back to Back Site-Directed Mutagenesis from Civic Bioscience combines a unique blend of enzymes intended to be used for the site-directed mutagenesis strategy utilizing the back-to-back primer design. The enzymatic activities of T4 Polynucleotide Kinase, T4 DNA Ligase and DMT enzyme (DpnI) are combined and achieve optimal performance in the enhanced 5x reactional buffer. In contrast to competitor products, our KLD Mix is compatible with any High-Fidelity DNA Polymerase that generates strictly blunt end PCR products.
Features of the KLD enzyme mix used for Site-Directed Mutagenesis
- Fast : 5-15 min room temperature reaction.
- Useful : compatible for point mutation, 80 base insertions and unlimited-size deletions
- Economical : No need to purchase 5′ phosphorylated oligos
- Efficient : 90-95% mutant colonies using regular 25-cycle PCR or a 10-cycle Fast & Steep PCR.
- Upgradable : combine the KLD enzyme mix with Fast & Steep PCR and DMT Competent Cells.
Note : When using site-directed mutagenesis strategies that utilize whole vector amplification, to ensure that no extra mutations are introduced in the backbone of the vector, it is strongly recommended to perform subcloning of the sequenced insert into a vector that hasn’t been amplified by PCR. Alternatively, the whole vector can be sequenced.
Recommendation : Use the Fast & Steep WVA protocol for site-directed mutagenesis to limit the number of PCR cycles to a minimum whule keeping the number of doublings very high. The risk of introducing undesired mutations drops drastically by at least 100 000-fold.
Content of the KLD Mix for Back-to-Back Site-Directed Mutagenesis
- KLD Mix : 10 ul / 20 ul / 50 ul
- KLD Mix 5x buffer : 30 ul / 50 ul / 120 ul
- Control 4 kb Amplicon : 5 ul / 5 ul / 10 ul
Manual and datasheet
10 rx, 20 rx, 50 rx
Ask a question about KLD Mix for Back-to-Back Site-Directed Mutagenesis
Product Reviews for KLD Mix for Back-to-Back Site-Directed Mutagenesis
More to Read
by Simon Roy | Sep 16th, 2019 | Latest News, Promotions | Promo code : civicbio4-50 1 per customer. Using this coupon will reduce Cashback Reward earning rate by 50%. Until Nov, 30th, 2019 Double your Cashback Rewards with any product purchase! + Cashback x1.5...
Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...
By purchasing any of TransBionovo's DNA Polymerase, you instantly benefit from our PCR Success Guarantee !
The Problem with Antibodies used for Research Antibodies used for Research in academic, industrial and therapeutic fields are universal reagents in molecular biology assays. Without them, the entire field of medical research could collapse. However, there are many...
GC-rich PCR: FastPfu FLY vs Q5® High-Fidelity DNA Polymerase GC-rich PCR can be difficult to achieve Our aim was to verify compare FastPfu FLY (TransBionovo) and Q5 (NEB) high-fidelity DNA Polymerase at amplifying a GC-rich sequence (human ARX; 79% GC) from purified...
What's the Detection Limit of Taq ? How many DNA copies from gDNA? There are MANY Taq DNA Polymerases out there on the market. Some are driven by strong marketing and others by strong performance. Our aim was to verify the Detection Limit of Taq DNA Polymerases in PCR...
Experimental dates range : 2018-05 to 2018-10 Published : September 22nd, 2019 by Simon Roy, Ph.D Extremely variable GC composition found in a 2.1 kb genomic DNA sequence coding for exon 1, an intron and exon 2 of the human TWIST1 gene DNA...
A huge THANK YOU for your support! MERCI encore INFINIMENT de supporter notre jeune compagnie! by Simon Roy | September 16th, 2019 | Company Posts, Latest NewsPrices for services vary case by case. 4rth anniversary promotions are coming soon! Let's CELEBRATE...
DNA Polymerases | EasyTaq | FastPfu | PCR | PCR Purification | Taq DNA PolymeraseSave on the Products that MatterOur already popular 222$ DUO Miniprep + Gel/PCR Purification now allows you to choose 2 x 300rx minipreps or 2x 300rx Gel/PCR purification. Already...