IPTG

Isopropylthio-β-galactoside (IPTG) is an effective inducer of β-galactosidase activity and it remains stable in bacteria metabolism. If IPTG is added together with X-gal into culture medium, based on the α-complementation of β-galactosidase, recombinant clones can be easily identified upon whether white colonies are observed. In addition, IPTG can also serve as an inducer of protein expression in lac or tac promoter -regulated expression vectors.

Applications

• IPTG is used together with X-gal in blue/white screening.
• Used as an inducer of protein expression under the control of lac or tac promoter.

Concentration

500 mM

Storage

at -20°C for six months

Manual and Datasheet:

IPTG