Human VTN (Vitronectin) ELISA Kit
The Human VTN ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VTN. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VTN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VTN. You can calculate the concentration of VTN in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Vitronectin
Abbreviation : VTN
Uniprot ID : P04004
Format : 96 rx
Reactivity : Human
ELISA configuration : Sandwich
Sensitivity : 1.875 ng/ml
Detection Range : 3.125 – 200 ng/ml
More information about this Human VTN (Vitronectin) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
---|
Reviews