Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) ELISA Kit – E-EL-H2371

Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) ELISA Kit

The Human UBA52 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UBA52. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for UBA52 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBA52, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of UBA52. You can calculate the concentration of UBA52 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1

Abbreviation : UBA52

Uniprot ID : P62987

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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