Human TTR (Transthyretin) ELISA Kit – E-EL-H2342

Human TTR (Transthyretin) ELISA Kit

The Human TTR ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TTR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TTR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TTR. You can calculate the concentration of TTR in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Transthyretin

Abbreviation : TTR

Uniprot ID : P02766

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 9.375 ng/ml

Detection Range : 15.625 – 1000 ng/ml

Manual & Datasheet

More information about this Human TTR (Transthyretin) ELISA Kit from Elabscience