Human TI-Ag (Thymus Independent Antigen) ELISA Kit – E-EL-H2145

Human TI-Ag (Thymus Independent Antigen) ELISA Kit

The Human TI-Ag ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TI-Ag. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human TI-Ag and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TI-Ag, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TI-Ag. You can calculate the concentration of Human TI-Ag in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Thymus Independent Antigen

Abbreviation : TI-Ag

Uniprot ID :

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Human TI-Ag (Thymus Independent Antigen) ELISA Kit from Elabscience