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Human SMPD1 Protein (His Tag) – PKSH030434

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Recombinant Human SMPD1 Protein (His Tag)

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as ASM (acid sphingomyelinase), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.


Synonym(s) : SMPD1


Species(s) : Human


Protein Expression Host : Baculovirus-Insect Cells


Protein Construction : A DNA sequence encoding the human SMPD1 (BAD93012.1) (Met1-Pro628) was expressed with a polyhistidine tag at the C-terminus.


Predicted N-terminal amino acid : Leu 47


Molecular weight/mass : The recombinant human SMPD1 consists of 593 amino acids and predicts a molecular mass of 66.3 kDa.


Protein Construction : A DNA sequence encoding the human SMPD1 (BAD93012.1) (Met1-Pro628) was expressed with a polyhistidine tag at the C-terminus.


Formulation : Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 25 % glycerol, pH 7.5


Purity : > 95 % as determined by SDS-PAGE.


Endotoxin level : < 1.0 EU per μg protein as determined by the LAL method.


Stability : Samples are stable for up to twelve months from date of receipt at -70°C


Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


Reconstitution : Please refer to the printed manual for detailed information.


Manual & Datasheet


Additional information

Format

10 ug, 50 ug

Species

Human

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