Human SMPD1 / ASM Protein (His tag) – PKSH030372

Recombinant Human SMPD1 / ASM Protein (His tag)

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as ASM (acid sphingomyelinase), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

Synonym(s) : ASM , NPD , SMPD1

Species(s) : Human

Protein Expression Host : Baculovirus-Insect Cells

Protein Construction : A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : His 62

Molecular weight/mass : The secreted recombinant human SMPD1 consists of 518 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.1% OGP, 10% glycerolcerol

Purity : > 94 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : Liquid. It is shipped out with blue ice.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet