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Human SEMA4D (Semaphorin 4D) ELISA Kit – E-EL-H2252

96 rx

797.00$ CAD

0 in stock (expect 7-14 days for delivery)

0 in stock (expect 7-14 days for delivery)

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SKU: E-EL-H2252 Category: Tags: , Brand:

Human SEMA4D (Semaphorin 4D) ELISA Kit

The Human SEMA4D ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4D. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA4D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA4D. You can calculate the concentration of Human SEMA4D in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Semaphorin 4D

Abbreviation : SEMA4D

Uniprot ID : Q92854

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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96 rx

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