Human PTPN11 (Protein Tyrosine Phosphatase, Non Receptor Type 11) ELISA Kit – E-EL-H2246

Human PTPN11 (Protein Tyrosine Phosphatase, Non Receptor Type 11) ELISA Kit

The Human PTPN11 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTPN11. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PTPN11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTPN11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTPN11. You can calculate the concentration of Human PTPN11 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Protein Tyrosine Phosphatase, Non Receptor Type 11

Abbreviation : PTPN11

Uniprot ID : Q06124

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Human PTPN11 (Protein Tyrosine Phosphatase, Non Receptor Type 11) ELISA Kit from Elabscience