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Human PKP1 (Plakophilin 1) ELISA Kit – E-EL-H2204

96 rx

797.00$ CAD

0 in stock (expect 7-14 days for delivery)

0 in stock (expect 7-14 days for delivery)

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SKU: E-EL-H2204 Category: Tags: , Brand:

Human PKP1 (Plakophilin 1) ELISA Kit

The Human PKP1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKP1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for PKP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PKP1. You can calculate the concentration of PKP1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Plakophilin 1

Abbreviation : PKP1

Uniprot ID : Q13835

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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96 rx

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